Walnut – Juglans Regia

Family: Juglandaceae

Part Used:


NOTE: These indications are only for use with embryonic plant stem cell tissues. Adult plants do not have the same constituents, actions or applications in most cases.

The Juglans regia is a large deciduous tree that is native to Southeast Europe, the Himalayas, and China. It grows 40 to 60 feet tall and has a broad, rounded open crown. The bark is thick and deeply furrowed on the trunk, producing a striking diamond shaped pattern, but smooth and grey on the younger branches. Its alternate, pinnately compound leaflets are 12 to 18 inches long, and the individual egg-shaped leaves are 5 inches long with finely serrated margins. Small, yellowish-green scented flowers bloom in June, followed by the ripening of its fruits in October. The fruits are brown corrugated nuts enclosed in green, semi-fleshy husks. Edible uses include oil, sap, seed, sweetener, and tea. Other uses include dye, herbicide, oil, paint, polish, repellent, tannin, and wood. The close-grained wood of this tree is heavy, hard, and durable and is valuable for furniture making, veneer, etc.

Other Uses:
Dye; Herbicide; Oil; Paint; Polish; Repellent; Tannin; Teeth; Wood.

A yellow dye is obtained from the green husks. It is green. The green nuts and the leaves are also used. The rind of unripe fruits is a good source of tannin.

A brown dye is obtained from the leaves and mature husks. It does not require a mordant, and it turns black if prepared in an iron pot. The dye is often used as a coloring and tonic for dark hair. The leaves and the husks can be dried for later use.

A golden-brown dye is obtained from the catkins in early summer. It does not require a mordant. A drying oil is obtained from the seed. It is used in soap making, paints, etc. It is not very stable and quickly goes rancid.

The nuts can be used as a wood polish. Simply crack open the shell and rub the kernel into the wood to release the oils.

Wipe off with a clean cloth.

The dried fruit rind is used to paint doors, window frames etc. (It probably protects the wood due to its tannin content).

The leaves contain juglone; this has been shown to have pesticidal and herbicidal properties. The crushed leaves are an insect repellent.

Juglone is also secreted from the roots of the tree; it has an inhibitory effect on the growth of many other plants.

The bark of the tree and the fruit rind are dried and used as a tooth cleaner. They can also be used fresh.

The wood of the Walnut is heavy, hard, durable, close grained, and seasons and polishes well. A very valuable timber tree, it is used for furniture making, veneer etc.

Back flower applications: Walnut allows one to make or adapt to major life changes and to fulfill ambitions free from the influence of others. For people who are affected by negative and pessimistic thinking from others; for those who worry about what other people think. These applications also apply to Plant Stem Cell Therapy.

Walnut - Juglans regia

The Brain is fixed inside the skull as in a walnut shell
a) Cerebral mass and brain of the nut
b) Magpie and thin peel of the nut
c) Tough skin of the peel coarse matter of the nut
d) Bony envelope and shell
e) The cutaneous fleshy sheathing

Abstracts of Published Research on Walnut – Juglans Regia:

1. Georgian Med News. 2009 May;(170):110-4.
Antidiabetic action of extract of Juglans regia L Dzhafarova RE, Garaev GSh, Dzhafarkulieva ZS.

2. Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2535-8.
Investigation on inhibition of HIV III B virus with extractions of Juglans regia Liu ZM, Wen RX, Ma HT, Yang YS, Wang XL, Lv XH, Li ZL.

3. Evid Based Complement Alternat Med. 2008 Dec;5(4):421-8. Epub 2007 May 17.
Maintaining a physiological blood glucose level with 'glucolevel', a combination of four anti-diabetes plants used in the traditional arab herbal medicine. Said O, Fulder S, Khalil K, Azaizeh H, Kassis E, Saad B.

4. Food Chem Toxicol. 2008 Jul;46(7):2326-31. Epub 2008 Mar 28.
Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Oliveira I, Sousa A, Ferreira IC, Bento A, Estevinho L, Pereira JA.

5. Phytother Res. 2008 Apr;22(4):557-9
Antimycobacterial activity of Juglans regia, Juglans mollis, Carya illinoensis and Bocconia frutescens. Cruz-Vega DE, Verde-Star MJ, Salinas-González N, Rosales-Hernández B, Estrada-García I, Mendez-Aragón P, Carranza-Rosales P, González-Garza MT, Castro-Garza J.

6. Food Chem Toxicol. 2008 Jun;46(6):2103-11. Epub 2008 Feb 8.
Bioactive properties and chemical composition of six walnut (Juglans regia L.) cultivars. Pereira JA, Oliveira I, Sousa A, Ferreira IC, Bento A, Estevinho L.


Au, B, Cr, Cu, Fe, I, K, Mb, Mg, Mn, Na, Ni, P, Se, Si, Su, Ti, V, Zn.

Vitamins and Minerals:

A, B-1, B-2, B-3, B-5, B-6, C, Calcium, E, Folacin.

Phytochemical Constituents:

3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, Adenosine, Adenine B-4, Alpha-Tocopherol, Ascorbic-Acid, Avicularin, Auxins (IAA), Beta-Carotene, Beta-Sitosterol, Brassinosteroids (BA), Campesterol, Catechin/Gallic Acid, Chlorine, Neo-chlorogenic acids, Cytokinins (CK), Delta-Tocopherol, 2 novel dicarboxylic acid derivatives, Ellagic-Acid, 3 new Ellagitannins Glansreginins A B, Fiber, Gamma lactones. Gamma-Tocopherol, Gibberellins (GA), Germacrene D, Hyperin, Juglone (5-hydroxy-1, 4-naphthagulone), Alpha-hydrojuglone (1, 4, 5-trihydroxynaphthalene) and its glycoside beta-hydrojuglone, Bisjuglone, Trijuglone, Regiolone, (Müller and Leistner, 1978; Talapatra et al., 1988). Kaempferol, L-arginine, Meristems plant stem cells (PSC), Polyunsaturated fatty acids Alpha linolenic (n-3) acid and Linoleic (n-6) acid, Methyl Palmitate (MP), Lutein, Melatonin, Myricetin, Myricitrin, Oleic Acid, Palmitic Acid, p-coumaric acid, Polyamines, Polyphenols, Quercetin; Quercetin 3-pentoside derivative, Quercetin 3-arabinoside, Quercetin 3-xyloside and Quercetin 3-rhamnoside, Salicylates (SA), Sakuranetin, Stearic Acid, Sterols, Stigmasterol, Zeaxanthin.

New dimeric hydrolysable tannins galloylglucose and ellagitannins Glansrins A–C Ellagitannins with a tergalloyl group.
Extremely resistant to the formation of aflatoxin by molds growing on the kernel. Gallic acid is the responsible phytochemical for inhibiting aflatoxigenesis by Aspergillus flavus.

Polyphenols, polyamines, contain an antioxidant compound called ellagic acid that supports the immune system and appears to have several anticancer properties. Contains juglone which is green in color and most extract are brown containing barely any juglone, which has demonstrated both antibacterial and antifungal properties.The fatty acids of walnuts are prone to oxidation (rancidity) but when fresh maceration it was shown to prevents oxidation from occurring.

Black walnut is considered to be an antiseptic, a germicide, a parasitic, and a laxative.

Sterols are naturally occurring plant compounds that are chemically similar to cholesterol. Sterols may play chemoprotecive and cardioprotective roles. Tannins are antibacterial, anticancer, antidiarrheic, antihepatotoxic, chelators, antihypertensive, antitumor, cancer preventive, and antiulcer.

Walnuts have favorable levels of total phenols relative to other foods reported to have high phenolic and antioxidant levels, when compared on a per serving basis. The number of servings of these other foods that would have to be consumed to equal the total phenolics in a dose of 30 drops walnut buds is as follows: 1 dose of walnut buds 30 drops = 2.2 servings red wine = 3.9 servings of milk chocolate = 4.6 servings of apple juice. One of the best sources of phenolic antioxidants, a-tocopherol and unsaturated fatty acids, in addition to other many other phytochemical constituents. Also the bioavailability is greater when taken in an extract form. They have higher concentrations of expected physiologic plasma levels than that of dietary supplement phenolics.
Fatty acids identified and their relative percentages in the embryonic buds analyzed
Palmitic acid(Rt= 11.47)
Linoleic acid (Rt=12.96)
Oleic acid (Rt=13.03)
Stearic acid (Rt=13.10)
Walnut – Juglans Regia (buds)
Hazel – Corylus Avellana (buds)
Sweet Chestnut – Castanea Vesca (buds)

Plant Stem Cell Therapy :
Polycrest all Walnut is a tree that does not grow in a forest; it developed by itself alone with light. Strongly aromatic inhibits the growth of other plants. Rebalances the flora of the colon. This bud is a Polycrest for Diabetes by normalizing the enzymes and insulin production also the secretion of pancreatic juice. Antiviral to Hepatitis C, Antibiotic (anti-streptococcus anti-staphylococcus), Anti-Parasitic, Anti-Fungal, Anti-Inflammatory. Chronic Suppurations of the mucous membranes; Dermatological and Pulmonary. Stimulates the production of immunoglobulin.

Endocrine System:

Acute & Chronic Pancreatitis, Hemorrhagic Pancreatitis, Juvenile or Adult onset Diabetes Obesity (by detoxifying the receptor sites, insulin production will become normal again),glucose-insulin homeostasis, Hypoglycemic. Walnut – Juglans Regia (buds) tannins and Olive – Olea Europaea (young shoots) oleuropein, are glucosidase inhibitors thus reducing the absorption of carbohydrates in the gut, Also the antioxidants in both plants exert hepatic-pancreatic cytoprotection and enhance the therapeutic efficacy of glucolevel synergistically to regulate glucose-homeostasis. Hyperhydrosis; excessive perspiration of the hands and feet. Helps stimulate the function of the thyroidbecause of its high content of Iodine.

References: May 17, 2007 Omar Said1,2, Stephen Fulder1, Khaled Khalil1, Hassan Azaizeh2, Eli Kassis4 and Bashar Saad2,3
1Antaki Center for Herbal Medicine Ltd Kfar Kana 16930, PO Box 2205, 2Research and Development Regional Center (affiliated with Haifa University, Haifa, Israel) - The Galilee Society, PO Box 437, Shefa Amr 20200, Israel, 3Faculty of Allied Medical Sciences, The Arab American University Jenin, PO Box 240, Jenin, Palestine and 4Sprunk-Jansen A/S, Strandvejen 100, 2900 Hellerup, Denmark

Infectious Diseases:

Contains 14 Antibacterial and 14 Antiviral 5 Anti-Yeast phytochemicals. Hepatitis C, Infected Eczema, Trichinosis, Candida Albican, Parasitic Infections with the association of Fig – Ficus Carica. Contains juglone, which has demonstrated both antibacterial and antifungal properties. The antimicrobial capacity was screened against Gram positive (Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans). Walnut buds selectively inhibited the growth of Gram positive bacteria, being B. cereus the most susceptible one (MIC 0.1mg/mL). Gram negative bacteria and fungi were resistant to the extracts. In the United Kingdom and United States Clostridium perfringens bacteria are the third most common cause of food-borne illness. Antimicrobial activity of Juglone (5-hydroxy-1,4-naphthoquinone) produced strong (+++) inhibition against Clostridium perfringens. Effective in expelling all kinds of intestinal worms. It is extensively used as vermifuge and the result is unfailing. Counteract parasitic and fungal infections.

Cardio Vascular System:

Arteritis, Varicose Ulcers. Believed to prevent Infarctus. Reduction of total cholesterol and low-density lipoprotein–cholesterol and some apoprotein response. Also decrease plasma triglyceride (TG) concentration by 17%. Walnut buds polyphenolics are effective inhibitors of in vitro plasma and LDL oxidation. The antioxidant polyphenolic content of walnut buds a potential contributor to the apparent antiatherogenic effect of walnut buds extracts. Walnuts were found to increase the elasticity of arteries by 64 per cent and to reduce cell adhesion molecules associated with hardening of the arteries by 20 per cent. walnut activated the nuclear transcription factor identified in human atherosclerotic plaques.

Inhibits the TNF-a-induced endothelial activation and expression of ICAM-1 and VCAM-1 adhesion molecules, at a concentration range of 10–200mg/ml or 0.2–7mmol MC equivalents which are within the reported in vitro concentration range of walnut extract shown to inhibit LDL oxidation. The inhibitory effect of walnut extract was higher at low concentrations (10 mg/ml) than at higher concentrations. 200 mg/ml), possibly due to the presence of multiple components present in the extract which may result in synergistic or antagonistic actions respectively.Ellagic acid, a major component of walnut methanolic extract, inhibited also the TNF-a-induced endothelial activation and expression ofintracellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) adhesion molecules, when tested at a concentration range of 0.1–10mM. Yu et al. demonstrated that ellagic acid (at 25–50mM) inhibited the IL-1b-induced endothelial activation and expression of ICAM-1 and VCAM-1 adhesion molecules. Of note, they showed that ellagic acid mediated its anti-inflammatory effects via modulation of NF-kB activity. The present results and those reported by Yu et al. imply that ellagic acid may confer the favorable effect of the walnut extract on endothelial function. In this study contained not only ellagic acid but other phenolic compounds such as gallic acid, catechin, caffeic acid and coumaric acid, which have been previously shown to inhibit endothelial activation and ICAM-1 and VCAM-1 expression. Cardioprotection by improving endothelial function.

ENT: Chronic sinusitis, superior to neomycin in treating otitis.

Musculoskeletal System:

Chronic evolutive Poly-Arthritis, Ankylosing Peri-Arthritis. The methanolic extract from J. regia has a high anti-inflammatory potential that can be attributed, partly, to ellagic acid and also on osteoblast function suggests that the incorporation of walnut buds extracts beneficial to the skeletal system by preventing osteoporosis.

Pulmonary System:

Bronchitis, Sinusitis, Allergies, Hyperimmune Syndrome, Anti-Scrofulous.

Uro Genital System:

Repeated infections.

Gi-Digestive Hepatology:

Mal-Absorption, Malnutrition, Dysbiosis, Diverticulosis, Abdominal Distension, Diarrhea post antibiotic therapy Colitis, Viral Diarrhea, Liver afflictions stimulate Kupffer cells (macrophages), Alcoholic Cirrhosis. Restores intestinal flora of the colon. Promotes healthy digestion and the breakdown of fats. Excellent in food poisoning against Clostridium perfringens. Organic Walnut buds extracts has been used in many native cultures to help the body reject that which doesn’t belong there.


Infected Eczema, Psoriasis, Pustular Psoriasis, Pustular Acne, Lupus erythematous, Collagenosis, Impetigo, Ringworm (external topical application), Leprosy, Scleroderma, Dermatomyositis. Used topically to treat scalp itching, peeling, and dandruff, sunburn and superficial burns, and as an adjunctive emollient and itch-relieving treatment in skin disorders (Bruneton, 1995). In India, walnut leaf decoction is used externally as a wash for malignant sores and pustules (Nadkarni, 1976). Having a lethal effect on mites an acaricidal compound due to borne fatty acid ester, methyl palmitate (MP), which produced strong acaricidal activity (62.8% mortality) at 1 mg 10 drops at 24 h. Based on a slide dip bioassay, 10 mg/ml MP provided 97.9% mortality against adults of Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae), whereas mortality against eggs was much lower (57.2%). Methyl Palmitate, an Acaricidal Compound Occurring in the embryonic Walnut buds and green husk.

Hematology Oncology:

juglone has shown tumor inhibition (e.g., Ehrlich ascites tumor) effects in mice (Okada et al., 1967). L-arginine (a precursor to nitric oxide) is also found in significant amounts in walnut buds. Ellagitannins with a tergalloyl group Glansrins A–C with superoxide dismutase (SOD)-like activity with EC50 21.4-190 μM and a remarkable radical scavenging effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (EC50 0.34–4.72 μM) also protect lipid from oxidation. A reactive oxygen species has been implicated in a range of human pathological diseases such asatherosclerosis and certain cancers. Helps detoxify toxins and harmful pathogens.

Neurological System:

inhibit and defibrillize amyloid beta protein—a feature of the amyloid plaque seen in the brains of patients with Alzheimer disease, can slow the progression of cognitive decline.


Exudation Blepharitis


Can be used a mouthwash for bacterial pockets. Antibacterial and stenghtens the gums.


Generally recognized as safe.

Toxicology in Animals:

The naphthaquinone juglone, present in all of the family Juglandaceae, is a known animal toxin. Laminitis.